The Catholic University of America

 

 

Dr. Greg Miller
Associate Professor, Biochemistry


Dr. Greg Miller - The Catholic University of America
 

                        B. Sc (Biochemistry                 Ph.D. (Biochemistry)            
                   The University of Western Ontario (London, Ontario, Canada)

                           Tel: (202) 319-4766             Email:  millergj@cua.edu
                                                                                          

Teaching
 

CHEM 109            General, Organic, and Biochemistry for the Health Sciences

CHEM 119            General, Organic, and Biochemistry Lab for the Health Sciences

CHEM 405            Science Communication

CHEM 471            Biochemistry I

CHEM 472            Biochemistry II

CHEM 493            Undergraduate Research (positions available)

CHEM 496            Biochemical Techniques

BIOL 544               Enzymology
 

Primary Research Interests

Structural biology 
Protein Chemistry
Enzymology
 
My lab combines biochemical and structural techniques to study kinases and phosphatases that produce soluble inositol phosphate (IP) and membrane-bound phosphatidylinositol (PI) signals in cells. Inositol-based signalling is ubiquitous in human cells and its dysregulation has been linked with disease states. Our goal is to understand how these kinases and phosphatases produce highly specific inositol signals and how they elicit their effects in cells. To do this, we study the structures of  IP- and PI- kinases and phosphatases, their catalytic mechanisms, and how their actions are regulated in the cell. We use x-ray crystallography to determine high-resolution structures of these enzymes and protein engineering to correlate the structure of each enzyme with its catalytic function and their downstream effects. Using these approaches we generate a clear understanding of the functions of these kinases and phosphatases and we also obtain high-resolution structures to facilitate inhibitor design efforts to target inositol-based signalling networks.

Publications

Gosein V and Miller GJ (2013). Roles of Phosphate Recognition in Inositol 1,3,4,5,6-
Pentakisphosphate 2-Kinase Substrate Binding and Activation. Journal of Biological
Sciences J Biol Chem. 2013 Jul 24.
http://www.ncbi.nlm.nih.gov/pubmed/23884422
 
Khan, SM, Sleno, R, Gors, S, Zybergold, P, Laverdure, JP, Labbé, Miller GJ, Hébert,
TE (2013). The expanding roles of Gβγ subunits in G protein-coupled receptor signaling
and drug action Pharmacol Rev 13, 545-77.
 
Gosein, V., Leung, T-F., Krajden, O., Miller, G.J. (2012). Structural basis for
substrate activation of inositol pentakisphosphate 2-kinase Protein Science 21(5), 737-42.
http://www.ncbi.nlm.nih.gov/pubmed/22362712
 
Lamba, P., Wang, Y., Tran, S., Ouspenskaia, T., Libasci, V., Hébert, T.E., Miller, G.J.,
Bernard, D.J. (2010). Activin A regulates porcine follicle-stimulating hormone β subunit
transcription via cooperative actions of SMADs and FOXL2. Endocrinology 151(11),
5456-67.
 
Quiniou, C., Sapieha, P., Lahaie, I., Hou, X., Brault, S., Beauchamp, M., Leduc, M.,
Rihakova, L., Joyal, J-S., Nadeau, S., Heveker, N., Lubell, W., Sennlaub, F., Gobeil, F.,
Miller, G., Pshezhetsky, A., Chemtob, S. (2008). Development of a novel non-competitive
antagonist of interleukin-1 receptor. Journal of immunology 180, 6977-6987.
 
Miller, G.J., Wilson, M.P., Majerus, P.W., and Hurley, J.H. (2005). Specificity
determinants in inositol polyphosphate synthesis: crystal structure of inositol 1,3,4-
trisphosphate 5/6-kinase. Molecular cell 18, 201-212.
 
Miller, G.J., and Hurley, J.H. (2004). Crystal structure of the catalytic core of inositol
1,4,5-trisphosphate 3-kinase. Molecular cell 15, 703-711.
 
Miller, G.J., Mattera, R., Bonifacino, J.S., and Hurley, J.H. (2003). Recognition of
accessory protein motifs by the gamma-adaptin ear domain of GGA3. Nature structural
biology 10, 599-606.
 
Hurley, J.H., Anderson, D.E., Beach, B., Canagarajah, B., Ho, Y.S., Jones, E., Miller,
G., Misra, S., Pearson, M., Saidi, L., et al. (2002). Structural genomics and signaling
domains. Trends in biochemical sciences 27, 48-53.
 
3. Misra, S., Miller, G.J., and Hurley, J.H. (2001). Recognizing phosphatidylinositol 3-
phosphate. Cell 107, 559-562.